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Related Experiment Videos

Autoreactivity in human IgG+ memory B cells.

Thomas Tiller1, Makoto Tsuiji, Sergey Yurasov

  • 1Max-Planck-Institute for Infection Biology, D-10117 Berlin, Germany.

Immunity
|February 20, 2007
PubMed
Summary
This summary is machine-generated.

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Most human B cells initially produce self-reactive antibodies, but checkpoints usually eliminate them. Surprisingly, IgG(+) memory B cells in healthy individuals frequently express these self-reactive antibodies, potentially linked to autoimmunity.

Area of Science:

  • Immunology
  • Autoimmunity
  • B cell biology

Background:

  • Nascent B cells often express autoreactive antibodies, but checkpoints typically remove them.
  • Despite rigorous selection, low-affinity self-reactive antibodies are found in healthy individuals.
  • The origin of these persistent self-reactive antibodies in normal serum is unclear.

Purpose of the Study:

  • To investigate the source of self-reactive antibodies found in healthy human serum.
  • To determine if IgG(+) memory B cells contribute to the pool of self-reactive antibodies.

Main Methods:

  • Cloning and expression of antibodies from circulating human IgG(+) memory B cells.
  • Analysis of antibody self-reactivity, including anti-nuclear antibodies.
  • Investigation of antibody generation through somatic hypermutation.

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Main Results:

  • Self-reactive antibodies, including anti-nuclear antibodies, were frequently detected in IgG(+) memory B cells from healthy donors.
  • A significant proportion of these self-reactive antibodies were generated de novo during B cell maturation.
  • Somatic hypermutation during the transition from naive to memory B cells appears to create these self-reactive antibodies.

Conclusions:

  • IgG(+) memory B cells in healthy individuals can express self-reactive antibodies.
  • The generation of self-reactive antibodies by memory B cells may occur through somatic hypermutation.
  • Deregulation of self-reactive IgG(+) memory B cells could be implicated in the development of autoimmunity.