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Related Experiment Videos

Infrared-based protein detection arrays for quantitative proteomics.

Christian Loebke1, Holger Sueltmann1, Christian Schmidt1

  • 1Division of Molecular Genome Analysis, German Cancer Research Center, Heidelberg, Germany.

Proteomics
|February 20, 2007
PubMed
Summary
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We developed a highly sensitive protein microarray assay (IPAQ) for detecting proteins in the femtogram range. This method enables quantitative proteomics and analysis of signaling networks for drug discovery.

Area of Science:

  • Biochemistry
  • Proteomics
  • Systems Biology

Background:

  • Current methods for analyzing signaling networks lack sensitivity and throughput.
  • This limits quantitative descriptions needed for systems biology and drug discovery.

Purpose of the Study:

  • To develop a highly sensitive protein microarray assay for quantitative protein detection.
  • To improve throughput for analyzing signaling networks in biological samples.

Main Methods:

  • Developed an improved protein microarray assay using near-infrared signal detection.
  • The assay, named IPAQ (infrared-based protein arrays with quantitative readout), detects proteins in the femtogram range.

Main Results:

  • IPAQ achieves high sensitivity, quantifying proteins from as few as 20,000 cells with a 5% error rate.

Related Experiment Videos

  • The assay can analyze up to 500 samples per microarray.
  • Protein abundance can be determined qualitatively and quantitatively (with recombinant protein).

    • Conclusions:

    • IPAQ offers a superior, sensitive approach for quantitative proteomics compared to existing technologies.
    • This method facilitates detailed analysis of signaling networks and crosstalk in biopsy samples for drug discovery.