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Bacteriophage Removal from Infected Salmonella Cultures
Published on: June 28, 2024
Simple colorimetric microplate test of phage lysis in Salmonella enterica
1United States Department of Agriculture, Agricultural Research Service, Waste Management, and Forage Research Unit, Mississippi State, Mississippi, 39762-5367, USA. mmclaughlin@ars.usda.gov
Abstract:
A simple microplate method, based on conversion of tetrazolium to formazan, was devised for rapidly assessing Salmonella survival after phage treatment. Results were easily interpretable. Monitoring with a microplate reader was useful, but not required. The method was used in defining phage-Salmonella interactions for selection of phage biocontrol cocktails.
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