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Updated: Jul 16, 2026
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Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
Coregulators: transducing signal from transcription to alternative splicing
Didier Auboeuf1, Eric Batsché, Martin Dutertre
1INSERM, U685/AVENIR, Centre G. Hayem, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75010 Paris, France. auboeuf@stlouis.inserm.fr
Trends in Endocrinology and Metabolism: TEM
|February 27, 2007
Summary
Transcriptional regulators control gene expression by influencing both transcription and alternative splicing. This study details how these regulators, particularly in steroid hormone pathways, impact exon content and gene message.
Area of Science:
- Molecular Biology
- Gene Regulation
- Cellular Signaling
Background:
- Cells modulate gene expression in response to external stimuli.
- Gene expression control occurs at transcriptional and post-transcriptional (pre-mRNA maturation) levels.
- Alternative splicing of pre-mRNAs alters exon content, changing the gene's message.
Purpose of the Study:
- To describe how transcriptional regulators and coregulators influence alternative splicing.
- To focus on genes regulated by steroid hormones.
- To discuss recent technical advances in studying stimulus-induced changes in exon content.
Main Methods:
- Review of existing literature on transcriptional regulation and alternative splicing.
- Analysis of mechanisms linking transcriptional control to splicing outcomes.
- Discussion of emerging technologies for transcriptomic analysis.
Main Results:
- Transcriptional regulators and coregulators directly impact alternative splicing patterns.
- Steroid hormone signaling pathways provide a model system for studying this linkage.
- New techniques enable detailed examination of how stimuli alter exon usage in gene transcripts.
Conclusions:
- Transcriptional and splicing control mechanisms are interconnected.
- Understanding this interplay is crucial for deciphering gene expression regulation.
- Advances in technology are enhancing our ability to investigate these complex processes.
Related Concept Videos
02:18Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
02:18Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
01:36What is Gene Expression?
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
01:42What is Gene Expression?
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
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01:23Regulation of Expression at Multiple Steps
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...