Related Experiment Video
Updated: Jul 16, 2026

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
Published on: July 5, 2017
[Microwave-assisted extraction of flavonoids in Mulberry leaf and root-bark]
Jing-jing Chen1, Xiang-rong Li
1College of Pharmaceutical Science, Zhejiang University, Hangzhou 310031, China.
Objective:
To extract flavonoids in Mulberry leaf and White Mulberry root-bark by microuave-assisted method.
Methods:
An orthogonal table was used to find the best procedure for microwave-assisted extraction (MAE) of flavonoids. The advantages of MAE were explored by comparing with the commonly used extraction methods. The different contents of flavonoids in Mulberry Leaf and Mulberry Root-bark were also compared.
Results:
The best condition for procedure was extracting 20 min at 60 degrees C with 12 times of 70% ethanol. Among all factors, the ethanol concentration was significant This experiment implied that flavonoids in Mulberry leaf were 9 times as that in Mulberry Root-bark probably. Moreover, the flavonoids extracted from Mulberry Leaf and White Mulberry root-bark by MAE were 55% and 32% more than that from commonly used extraction method individually.
Conclusion:
MAE is advantageous than commonly used extraction method. It can be used for batch production.
