Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Polarity of the Cytoskeleton

Polarity of the Cytoskeleton

The intrinsic polarity of cells can be primarily attributed to two factors- i) the asymmetric accumulation of mobile components such are regulatory molecules and subcellular components across the cell and ii) the orientation of polar cytoskeletal filaments that make up the cytoskeletal networks, specifically microfilaments, and microtubules arranged along the axis of polarity. Interactions between the cytoskeletal filaments are crucial for the establishment and maintenance of the polar nature...

Introduction to the Cytoskeleton

Introduction to the Cytoskeleton

Overview of the Cytoskeleton
The cytoskeleton is a network of protein filaments present within the cell, having three distinct filaments ̶ microfilaments, microtubules, and intermediate filaments. Each has characteristic features that distinguish them, including the dynamics of their assembly and disassembly, mechanical properties, polarity, and the type of molecular motors associated with them. Earlier, they were thought to be present only in eukaryotic cells; however, their...

Studying the Cytoskeleton

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

Respiratory Regulation of Acid-Base Balance

Respiratory Regulation of Acid-Base Balance

Respiratory compensation is a vital physiological process that stabilizes blood plasma pH by regulating the partial pressure of carbon dioxide (PCO2), a key determinant of pH levels. Most carbon dioxide in the blood dissolves and converts into carbonic acid (H2CO3). It dissociates into hydrogen ions (H+) and bicarbonate ions (HCO3⁻). There is also an inverse relationship between PCO2 and pH.
When carbon dioxide levels increase in the blood, more H+ and HCO3⁻ are...

Renal Regulation of Acid-Base Balance

Renal Regulation of Acid-Base Balance

Metabolic reactions in the body produce nonvolatile acids, such as sulfuric acid, which generate an acid load of approximately 1 mEq of H+ per kilogram of body weight daily. Excreting H+ in the urine is essential to balance this acid load.
In the kidneys, cells within the proximal convoluted tubules (PCT) and the collecting ducts secrete hydrogen ions (H+) into the tubular fluid. Specifically, in the PCT, Na+/H+ antiporters secrete H+ while reabsorbing Na+.
However, the intercalated cells in...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Assembly of Microtubule Tactoids Through Condensates of PRC1 Constructs.

Biomolecules·2026

Same author

Vimentin molecular linkages with nesprin-3 enhance nuclear deformations by cell geometric constraints.

Scientific reports·2026

Same author

Mitotically Driven Cytoskeletal Reorganization Governs Zebrafish Left-Right Organizer Detachment from EVL and Lumen Morphogenesis.

bioRxiv : the preprint server for biology·2026

Same author

Insights into the zebrafish left-right organizer's centrosomes and cilia via volume electron microscopy.

Biology open·2026

Same author

Functionally Essential and Structurally Diverse: Insights into the zebrafish Left-Right Organizer's Cilia via Optogenetic IFT88 Perturbation and Volume Electron Microscopy.

bioRxiv : the preprint server for biology·2025

Same author

Dynamic forces drive cell and organ morphology changes during embryonic development.

Proceedings of the National Academy of Sciences of the United States of America·2025

Same journal

Investigating transcription factor dynamics in health and disease using FRAP.

FEBS letters·2026

Same journal

Regulation of CFTR stability at the plasma membrane-Mechanisms and therapeutic opportunities in cystic fibrosis.

FEBS letters·2026

Same journal

Identification of a Shiga toxin A-derived peptide internalized into Gb3 receptor-bearing cells via interaction with the Shiga toxin B subunit.

FEBS letters·2026

Same journal

The dual role of lectins in cancer-immunotherapy tools and therapeutic targets.

FEBS letters·2026

Same journal

Decoding the dynamic extracellular matrix in cancer-3D models and bioscaffolds rewire the rules of tumor progression.

FEBS letters·2026

Same journal

Extending the classical sequence-structure-function paradigm through protein dynamics and context-dependent behavior.

FEBS letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 5, 2026

In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles

In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles

Published on: August 25, 2022

Regulating cytoskeleton-based vesicle motility.

Heidi Hehnly¹, Mark Stamnes

¹Department of Molecular Physiology and Biophysics, Roy J. and Lucille A. Carver College of Medicine, The University of Iowa, Iowa City, IA 52242, USA

|March 6, 2007

Summary

This summary is machine-generated.

Vesicle transport relies on coordinating coat assembly and cargo selection with movement. Cargo proteins influence cytoskeletal dynamics and motor proteins, potentially directing vesicle motility and targeting.

More Related Videos

Visualizing Bacterial Motility Based on a Color Reaction

Visualizing Bacterial Motility Based on a Color Reaction

Published on: February 15, 2022

Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles

Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles

Published on: November 10, 2021

Related Experiment Videos

Last Updated: Feb 5, 2026

In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles

In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles

Published on: August 25, 2022

Visualizing Bacterial Motility Based on a Color Reaction

Visualizing Bacterial Motility Based on a Color Reaction

Published on: February 15, 2022

Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles

Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles

Published on: November 10, 2021

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Vesicular transport is crucial for intracellular communication, requiring coordinated assembly of coat complexes and cargo selection.
Efficient translocation of vesicles from donor to acceptor compartments is essential for cellular function.
Understanding the link between vesicle components and motility machinery is key to deciphering intracellular trafficking.

Purpose of the Study:

To review recent advancements in understanding the molecular mechanisms connecting transport vesicles to cytoskeleton-mediated motility.
To highlight the role of vesicle cargo in influencing intracellular transport dynamics.
To explore how cargo composition impacts vesicle targeting and movement.

Main Methods:

Literature review of recent research on vesicular transport and cytoskeleton interactions.
Analysis of molecular mechanisms governing vesicle motility.
Synthesis of findings on cargo protein influence on cytoskeletal dynamics and motor proteins.

Main Results:

Emerging evidence suggests vesicle cargo proteins directly or indirectly affect cytoskeletal dynamics.
Cargo proteins interact with coat proteins, influencing the machinery for vesicle movement.
Vesicle cargo composition appears to play a role in directing intracellular motility and targeting.

Conclusions:

Vesicle cargo composition is an important factor in regulating intracellular transport.
Cargo proteins actively participate in modulating cytoskeletal dynamics and motor protein function.
This cargo-mediated regulation provides a mechanism for precise vesicle targeting and motility.