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Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
Use of colorimetric method for evaluation of LPS of different structure
1Laboratory of Molecular Biomedicine, Institute of Fundamental Problems of Biology, Russian Academy of Sciences, Pushchino. zusvet@rambler.ru
Bulletin of Experimental Biology and Medicine
|March 17, 2007
Abstract:
We studied the possibility of using the colorimetric method with carbocyanin dye for quantitative measurements of LPS of different structure in aqueous solutions. The absorption spectra (intensity and position of absorption peaks) of LPS-carbocyanin complexes depend not only on the presence of polysaccharide fragment in LPS structure, but also on the core structure.
