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Updated: Jul 16, 2026
09:45
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
Published on: February 9, 2012
Rapid hyperspectral fluorescence lifetime imaging
Pieter De Beule1, Dylan M Owen, Hugh B Manning
1Department of Physics, Imperial College London, South Kensington Campus, London, SW7 2AZ, United Kingdom.
Microscopy Research and Technique
|March 17, 2007
Abstract:
We report a rapid hyperspectral fluorescence lifetime imaging (FLIM) instrument that exploits high-speed FLIM technology in a line-scanning microscope. We demonstrate the acquisition of whole-field optically sectioned hyperspectral fluorescence lifetime image stacks (with 32 spectral bins) in less than 40 s and illustrate its application to unstained biological tissue.
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