Related Experiment Video
Updated: Jul 16, 2026
09:07
Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
Published on: August 22, 2019
Strategies for selective priming of memory B cells
Vanitha S Raman1, Evan F Lind, Micah J Benson
1Department of Microbiology & Immunology, Dartmouth Medical School, Lebanon, NH 03756, USA.
Immunology Letters
|March 22, 2007
Summary
New vaccine strategies can generate antigen-specific memory B cells (Bmem) without initial plasma cell (PC) production. This primes the immune system for a robust response upon subsequent exposure, enhancing protection.
Area of Science:
- Immunology
- Vaccinology
- Cellular Biology
Background:
- Long-term humoral immunity depends on memory B cells (Bmem) and long-lived plasma cells (PCs).
- Current vaccines typically induce both Bmem and PCs simultaneously.
- The differentiation pathways for Bmem and PCs are not fully understood.
Purpose of the Study:
- To investigate if Toll-like receptor (TLR) agonists can selectively induce Bmem expansion without PC generation.
- To explore novel vaccine strategies for priming immune responses.
Main Methods:
- Co-administration of soluble protein antigen (sAg) with TLR 3 or TLR 9 agonists.
- Analysis of germinal center (GC) formation, Bmem, and long-lived bone marrow (BM) PCs.
- Assessment of immune response upon re-challenge.
Main Results:
- TLR agonists with sAg induced GC formation and antigen-specific Bmem.
- Long-lived BM PC generation was not substantially induced.
- Re-challenge led to high PC levels and significant antigen-specific IgG titers.
Conclusions:
- Specific TLR agonists can decouple Bmem expansion from long-lived PC generation.
- Vaccines can be designed to prime hosts without immediate high antibody production.
- This approach offers a new strategy for developing effective vaccines.
More Related Videos
Related Concept Videos
01:23Cells of the Adaptive Immune Response
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
01:24Implicit Memories
Implicit memories, also known as non-declarative memories, are long-term memories that function outside of conscious awareness. These memories influence behavior and skills without explicit knowledge. This type of memory is evident in tasks like playing tennis, snowboarding, and texting. Implicit memory has three subsystems: procedural memory, conditioning, and priming. This type of memory is essential in various activities, from everyday tasks to specialized skills.
One key aspect of implicit...
One key aspect of implicit...
01:22Chunking and Rehearsal in Sensory Memory
Improving short-term memory can be achieved through techniques like chunking and rehearsal. Chunking involves organizing information into larger, more manageable units. This technique is particularly useful for information that exceeds the typical memory span of between five and nine items. For instance, logging into an online account with a password like "ta89vq0179gz" involves grouping letters and numbers into three chunks—ta89, vq01, and 79gz. It makes large amounts of information more...
01:24B Cell Activation and Differentiation
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
01:22T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
01:03Serial Position Effect
The serial position effect is a cognitive phenomenon where individuals are more likely to recall the first and last items in a list compared to those in the middle. This effect is divided into the primacy effect and the recency effect. The primacy effect is observed when the initial items in a list are remembered better. This occurs because these items are rehearsed more frequently or receive more elaborative processing, allowing them to be encoded into long-term memory more effectively. For...