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Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
Published on: March 7, 2018
Localized surface plasmon coupled fluorescence fiber-optic biosensor with gold nanoparticles
Bao-Yu Hsieh1, Ying-Feng Chang, Ming-Yaw Ng
1Institute of Biophotonics, Faculty of Life Sciences, National Yang Ming University, Taipei, Taiwan 112, Institute of Optical Sciences, National Central University, Jung-Li, Taiwan 320.
Analytical Chemistry
|March 24, 2007
Summary
A new fiber-optic biosensor uses localized surface plasmon coupled fluorescence (LSPCF) for highly sensitive detection. This localized surface plasmon coupled fluorescence biosensor can detect mouse immunoglobulin G (IgG) down to 1 pg/mL.
Area of Science:
- Biomedical Engineering
- Optoelectronics
- Nanotechnology
Background:
- Fiber-optic biosensors offer sensitive detection methods.
- Localized Surface Plasmon Resonance (LSPR) enhances optical sensing.
- Fluorescence detection provides high signal-to-noise ratios.
Purpose of the Study:
- To develop a novel fiber-optic biosensor utilizing a localized surface plasmon coupled fluorescence (LSPCF) system.
- To achieve highly sensitive detection of biomolecules, specifically mouse immunoglobulin G (IgG).
- To investigate the amplification effects of gold nanoparticles (GNPs) on fluorescence intensity.
Main Methods:
- Fabrication of a fiber-optic biosensor with a biomolecular complex in a sandwich format.
- Immobilization of a
complex on an optical fiber surface. - Excitation of LSPCF using the evanescent field and detection via a photomultiplier tube.
Main Results:
- The developed LSPCF biosensor successfully detected mouse immunoglobulin G (IgG).
- A limit of detection as low as 1 pg/mL (7 fM) for mouse IgG was achieved.
- Gold nanoparticle coupling significantly amplified the LSPCF intensity, confirming high sensitivity.
Conclusions:
- The novel fiber-optic biosensor based on LSPCF demonstrates very high sensitivity.
- This technology holds promise for sensitive and efficient biomolecular detection.
- The integration of LSPR and fluorescence in a fiber-optic platform enhances biosensing capabilities.
