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Updated: Jul 10, 2026

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
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Systemic RNAi in Caenorhabditis elegans.

C P Hunter1, W M Winston, C Molodowitch

  • 1Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.

Cold Spring Harbor Symposia on Quantitative Biology
|March 27, 2007
PubMed
Summary

Scientists identified SID-1 and SID-2 proteins crucial for RNA interference (RNAi) spreading in C. elegans. SID-1 transports double-stranded RNA (dsRNA) between cells, while SID-2 acts as a gut receptor for environmental dsRNA uptake.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • RNA interference (RNAi) is a gene silencing mechanism.
  • In *C. elegans*, RNAi spreads systemically and can be inherited.
  • Mechanisms of double-stranded RNA (dsRNA) transport are key to understanding RNAi efficacy.

Purpose of the Study:

  • To identify proteins responsible for the systemic spread of RNAi in *C. elegans*.
  • To elucidate the roles of these proteins in dsRNA transport into and between cells.

Main Methods:

  • Genetic screens to identify factors involved in RNAi spreading.
  • Protein structure and localization studies.
  • Functional characterization of identified proteins in dsRNA uptake and transport.

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Last Updated: Jul 10, 2026

RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
09:40

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Published on: February 13, 2012

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Main Results:

  • Two proteins, SID-1 and SID-2, were identified as essential for RNAi spreading.
  • SID-1 is a transmembrane protein facilitating dsRNA transport across cell membranes.
  • SID-2 is a gut-localized transmembrane protein acting as a receptor for environmental dsRNA uptake.

Conclusions:

  • SID-1 and SID-2 are critical mediators of dsRNA transport, enabling systemic RNAi in *C. elegans*.
  • SID-1 homologs are conserved in many animals, suggesting a fundamental role in dsRNA handling.
  • SID-2 exhibits unique properties in *C. elegans*, potentially representing a novel dsRNA uptake mechanism.