Related Experiment Video
Updated: Jul 16, 2026

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Circular dichroism spectra of DNA hairpins studied by the green function method
D Balamurugan1, Frederick D Lewis, Alexander L Burin
1Department of Chemistry, Tulane University, New Orleans, Louisiana 70118, USA.
Abstract:
The circular dichroism (CD) spectrum of a large biological molecule represented as an array of interacting chromophores is investigated. The configuration-averaged Green function formalism is developed to describe the CD and absorption spectra. The perturbation theory expansion is derived for absorption and CD spectra in the case of strong interaction of chromophores with their environment (solvent and/or internal dynamics) compared to their interaction with each other. We apply this formalism to study CD spectra of DNA hairpins.
More Related Videos
Related Concept Videos
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR
Single-Strand DNA Binding Proteins
¹H NMR: Interpreting Distorted and Overlapping Signals
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
¹³C NMR: ¹H–¹³C Decoupling
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...

