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Hydrogen peroxide biosensor based on hemoglobin modified zirconia nanoparticles-grafted collagen matrix
Shuizhen Zong1, Yong Cao, Yuming Zhou
1MOE Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China; Department of Chemistry, Changshu Institute of Technology, Changshu 215500, PR China.
A new biosensor using zirconia-grafted collagen immobilizes hemoglobin for detecting hydrogen peroxide (H2O2). This reagentless sensor offers high sensitivity and fast response, enabling practical sample analysis.
Area of Science:
- Biomaterials Science
- Electrochemistry
- Biosensor Technology
Background:
- Hemoglobin (Hb) immobilization is crucial for developing efficient biosensors.
- Developing reagentless biosensors with high sensitivity and stability remains a challenge.
Purpose of the Study:
- To propose a novel method for hemoglobin immobilization using a zirconia-grafted collagen scaffold.
- To develop a reagentless biosensor for hydrogen peroxide (H2O2) detection.
Main Methods:
- Immobilization of hemoglobin onto a biocompatible zirconia-grafted collagen tri-helix scaffold.
- Characterization using UV-vis, FT-IR, SEM, and electrochemical techniques.
- Construction and electrochemical evaluation of the H2O2 biosensor.
Main Results:
- The immobilized Hb exhibited excellent direct electrochemistry and electrocatalytic activity towards H2O2 reduction.
- The biosensor demonstrated a wide detection range (0.8–132 µM), low limit of detection (0.12 µM), and fast response (<5 s).
- The biosensor showed good affinity (apparent Michaelis-Menten constant of 0.026 mM), stability, and reproducibility.
Conclusions:
- Zirconia-grafted collagen serves as an effective matrix for protein immobilization and biosensor preparation.
- The developed reagentless biosensor is suitable for monitoring H2O2 in practical samples with satisfactory results.
