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Updated: Jul 15, 2026

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors
Published on: October 30, 2016
Construction of mouse adenovirus type 1 mutants
Angela N Cauthen1, Amanda R Welton, Katherine R Spindler
1Department of Natural Sciences, Clayton College and State University, Morrow, GA, USA.
Abstract:
Mouse adenovirus provides a model for studying adenovirus pathogenesis in the natural host. The ability to make viral mutants allows the investigation of specific mouse adenoviral gene contributions to virus-host interactions. Methods for propagation and titration of wild-type mouse adenovirus, production of viral DNA and viral DNA-protein complex, and transfection of mouse cells to obtain mouse adenovirus mutants are described in this chapter. Plaque purification, propagation, and titration of the mutant viruses are also presented.
