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Pupal and Adult Injections for RNAi and CRISPR Gene Editing in Nasonia vitripennis
Published on: December 4, 2020
A method for parental RNA interference in the wasp Nasonia vitripennis
Jeremy A Lynch1, Claude Desplan
1Department of Biology, New York University, 100 Washington Square East, New York, New York 10003, USA.
Nature Protocols
|April 5, 2007
Summary
Parental RNA interference (pRNAi) in the model organism Nasonia vitripennis allows researchers to study gene function by examining offspring for developmental defects. This efficient technique can elucidate gene roles in as little as two weeks.
Area of Science:
- Genetics
- Developmental Biology
- Entomology
Background:
- Nasonia vitripennis is a valuable model organism for biological research due to its tractability and sequenced genome.
- Effective methods for manipulating gene function are crucial for maximizing the utility of N. vitripennis.
Purpose of the Study:
- To describe a protocol for parental RNA interference (pRNAi) in Nasonia vitripennis.
- To enable the study of gene function in the progeny of manipulated wasps.
Main Methods:
- Injection of double-stranded RNA into N. vitripennis pupae.
- Allowing injected wasps to mature and reproduce.
- Examining the resulting progeny for developmental abnormalities.
Main Results:
- The pRNAi technique has successfully elucidated the function of numerous genes in N. vitripennis.
- The protocol is adaptable for studying gene function beyond embryonic patterning.
- Experiments can be completed rapidly, within approximately two weeks.
Conclusions:
- Parental RNA interference is an effective and efficient method for functional gene analysis in Nasonia vitripennis.
- This technique significantly enhances the utility of N. vitripennis as a model organism for genetic studies.
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