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Updated: Jul 15, 2026
DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat
Published on: February 27, 2014
Chemically induced mouse models of intestinal inflammation
Stefan Wirtz1, Clemens Neufert, Benno Weigmann
1Laboratory of Immunology, I Medical Clinic, University of Mainz, Mainz, Germany.
This study details protocols for chemically inducing intestinal inflammation in mice, crucial for understanding human inflammatory bowel diseases like Crohn's disease and ulcerative colitis.
Area of Science:
- Gastroenterology and Immunology
- Animal Models of Disease
Background:
- Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, significantly impacts human health.
- Animal models are essential for elucidating IBD pathogenesis.
Purpose of the Study:
- To provide standardized protocols for establishing chemically induced murine models of intestinal inflammation.
- To detail methods for 2,4,6-trinitrobenzene sulfonic acid (TNBS), oxazolone, and dextran sodium sulfate (DSS) colitis models.
Main Methods:
- Intrarectal administration of TNBS or oxazolone to induce T-cell-mediated colitis.
- Administration of DSS in drinking water for acute or chronic colitis, targeting colonic epithelial cells.
Main Results:
- Established protocols for TNBS, oxazolone, and acute/chronic DSS colitis models.
- Hapten and acute DSS models are completed within ~2 weeks.
- Chronic DSS colitis protocol requires ~2 months.
Conclusions:
- These chemically induced murine models offer valuable tools for IBD research.
- Standardized protocols facilitate reproducible studies on intestinal inflammation and IBD pathogenesis.
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