Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Basic protocols for zebrafish cell lines: maintenance and transfection.

Daniela Vallone1, Cristina Santoriello, Srinivas Babu Gondi

  • 1Department of Genetics, Max-Planck Institut für Entwicklungsbiologie, Tübingen, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|April 10, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A panel of medaka isogenic lines suggests individual, seasonal, and sexual genetic variation of <i>bdnf</i> gene expression in the brain.

Frontiers in behavioral neuroscience·2026
Same author

Whole-transcriptome insights into light-responsive non-coding RNA networks regulating circadian clock and DNA repair in zebrafish.

Functional & integrative genomics·2026
Same author

Developmental exposure to environmental levels of sulfamethoxazole (SMX) leads to hyperactivity in zebrafish larvae by inducing oxidative stress and calcium dyshomeostasis.

Journal of hazardous materials·2026
Same author

6-4 photolyase differentially modulates transcription in the vertebrate circadian clock.

PLoS genetics·2025
Same author

Light-Induced Transcription in Zebrafish Targets Mitochondrial Function and Heme Metabolism.

Antioxidants (Basel, Switzerland)·2025
Same author

A simple and rapid shaking-based assay to genotype live, early developmental stage zebrafish embryos.

Laboratory animals·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Zebrafish cell lines offer a simplified model for studying circadian clocks, exhibiting direct light entrainment and easy culture conditions. These cells enable high-throughput monitoring of clock gene expression using bioluminescence assays.

Area of Science:

  • Comparative genomics
  • Chronobiology
  • Cell biology

Background:

  • Vertebrate circadian clock regulation is crucial for physiological processes.
  • Mammalian cell lines require complex treatments to exhibit circadian rhythms.
  • Zebrafish embryos provide a promising source for novel cell culture models.

Purpose of the Study:

  • To establish and optimize zebrafish embryonic cell cultures for circadian clock research.
  • To develop protocols for efficient transfection and reporter gene assays in these cells.
  • To provide a simplified, robust system for studying circadian clock gene expression.

Main Methods:

  • Derivation and maintenance of zebrafish embryonic cell cultures.
  • Optimization of transient and stable transfection protocols.

Related Experiment Videos

  • Luciferase reporter construct introduction for monitoring clock gene expression.
  • Bioluminescence assays for high-throughput analysis of circadian rhythms.

    • Main Results:

    • Zebrafish cell lines display directly light-entrainable circadian rhythms of clock gene expression.
    • Culture conditions are simplified: room temperature, long viability at confluence, no CO2 required.
    • Successful introduction of luciferase reporters allows in vivo monitoring of clock gene activity.
    • The bioluminescence assay is suitable for high-throughput screening.

    Conclusions:

    • Zebrafish cell lines represent a powerful and accessible tool for circadian biology research.
    • These cells overcome limitations of mammalian cell lines, offering ease of culture and direct light responsiveness.
    • The described methods facilitate high-throughput investigation of circadian clock mechanisms.