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Updated: Jul 15, 2026
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
Published on: March 8, 2012
[Association between human papillomavirus (HPV) type 16 infection and E6/E7 gene variant and the cervical lesions in
Ying-jie Yang1, Jian Zhao, Qin-ping Liao
1Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing 100034, China.
Objective:
To investigate the association between HPV16 infection, E6/E7 variations and the cervical lesions.
Methods:
HPV subtypes were detected by using flow-through hybridization technique, E6/E7 gene was extracted from cervical lesions in 80 patients with HPV16 infection, PCR amplified, cloned into plasmid pMD18-T and sequenced.
Results:
HPV 16 was the most common type which accounted for 33.3% (154/463), the HPV16 infection rates increased with the severity of cervical lesions (P < 0.05). Totally in 72 cases the complete E6 and E7 regions were successfully sequenced, the DNA mutation rate of E6/E7 was 88.9% (64/72). A mutation, E6-D32E (T96G) coincided with a specific type of E7 mutation, N29S (A86G). D32E/N29S mutation rate was 38.9% (28/72), the detection rate increased with the severity of cervical lesions (P < 0.05).
Conclusion:
HPV 16 was the most common type in women with cervical lesions in Beijing, D32E/N29S variant associated with the cervical lesions.
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