Related Experiment Video
Updated: Jul 15, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
Combined supercritical fluid chromatographic methods for the characterization of octadecylsiloxane-bonded stationary
1Groupe de Chimie Analytique de Paris-Sud, EA 4041, IUT d'Orsay, 91400 Orsay, France. eric.lesellier@univ-orleans.fr
Abstract:
In this paper, we present a combination of a key-solute test based on retention and separation factors of large probe solutes (carotenoid pigments) and a quantitative structure-retention relationship analysis based on the retention factors of small probe solutes (aromatic compounds) to investigate the different chromatographic behavior of octadecylsiloxane-bonded stationary phases of all sorts: classical, protected against silanophilic interactions or not, containing polar groups (endcapping groups or embedded groups). Varied chemometric methods are used to enlighten the differences between the 27 phases tested. The results indicate that the two approaches chosen (carotenoid test and solvation parameter model) are complementary and provide precise information on the chromatographic behavior of ODS phases.
Related Concept Videos
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Chromatographic Methods: Classification
Chromatographic techniques are typically named by...
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
