Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microbiological contamination in stem cell cultures.

Fernando Cobo1, José Luis Cortés, Carmen Cabrera

  • 1Stem Cell Bank of Andalucía (Spanish Central Node), Hospital Universitario Virgen de las Nieves, Avda Fuerzas Armadas 2, 18014 Granada, Spain. fernando.cobo.sspa@juntadeandalucia.es

Cell Biology International
|April 25, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Temocillin versus carbapenems for bacteraemia due to third-generation cephalosporin-resistant Enterobacterales in Spain (ASTARTÉ): a multicentre, phase 3, open-label, non-inferiority, randomised clinical trial.

Lancet (London, England)·2026
Same author

"Un Dia de Conversación con Promotoras de Tejas": A Statewide, Spanish-Language CHW Conference in Texas and Its Impact on Mental Health.

Issues in mental health nursing·2026
Same author

Metatranscriptomic analysis of the microbiota of tumor tissue in colon cancer.

Microbiome·2026
Same author

Extreme heat reduces and reshapes urban mobility.

PNAS nexus·2026
Same author

In vitro susceptibility testing of cefiderocol in different media of Mueller-Hinton agar.

Enfermedades infecciosas y microbiologia clinica (English ed.)·2026
Same author

In vitro activity of eravacycline against anaerobic bacteria isolated from clinically significant samples.

Enfermedades infecciosas y microbiologia clinica (English ed.)·2026

Microbial contamination is a significant biosafety concern in cell therapy and regenerative medicine. This study found 12% of stem cell lines were contaminated, with Mycoplasma species being a key issue.

Area of Science:

  • Cellular biology
  • Microbiology
  • Regenerative medicine

Background:

  • Cell therapy and regenerative medicine offer novel therapeutic approaches.
  • Biosafety concerns, particularly microbial contamination, are critical for these treatments.
  • Bacteria, yeast, and fungi are common contaminants in cell products.

Purpose of the Study:

  • To assess the prevalence of microbial contamination in stem cell lines.
  • To identify common contaminants in cell therapy products.
  • To emphasize the importance of routine microbiological screening in stem cell banking.

Main Methods:

  • Analysis of 32 stem cell and feeder cell lines.
  • Microbiological screening for bacteria, yeast, and fungi.
  • Identification of specific contaminant types.

Related Experiment Videos

Main Results:

  • 19 out of 32 cell passages (12%) showed contamination.
  • Gram-positive cocci and Mycoplasma species were the most frequent contaminants.
  • Mycoplasma contamination was identified in 4% of the analyzed cell lines.

Conclusions:

  • Microbial contamination poses a significant risk in cell therapy and regenerative medicine.
  • Routine microbiological controls are essential for stem cell banks.
  • Preventing contamination ensures the safety and efficacy of cell-based therapies.