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Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
Published on: October 16, 2018
Replication-competent HIV strains infect HIV controllers despite undetectable viremia (ANRS EP36 study)
Aurelia Lamine1, Anne Caumont-Sarcos, Marie-Laure Chaix
1INSERM U802, Paris XI University, Bicêtre, France.
Abstract:
The replicative potential of HIV-1 strains in a well-characterized group of eight HIV controllers was investigated. Replication-competent viruses were detected in CD4 T-cell co-culture supernatants from all HIV controllers. The phylogenetic analysis of C2V4 suggested viral evolution or co-infection or superinfection in two out of the four patients analysed. The vif and vpr genes were normal. Infection with HIV-1 variants with attenuated replicative capacity cannot be a general factor accounting for undetectable viraemia in HIV controllers.
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