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Updated: Jul 15, 2026
09:27
Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Cofactor regeneration for sustainable enzymatic biosynthesis
1National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China.
Biotechnology Advances
|April 27, 2007
Summary
Efficiently regenerating expensive enzyme cofactors like NAD and NADP is crucial for industrial biotechnology. This review focuses on methods for cofactor retention in immobilized enzyme systems, specifically membrane entrapment and solid-attachment.
Area of Science:
- Biocatalysis and Industrial Biotechnology
- Enzyme Engineering
- Green Chemistry
Background:
- Oxidoreductases are valuable biocatalysts for synthesis, biosensing, and pollutant degradation.
- High cost of essential cofactors (NAD, NADP) limits industrial application of oxidoreductases.
- Cofactor regeneration is critical for economic viability, especially with immobilized enzymes.
Purpose of the Study:
- To review methods for cofactor retention in immobilized enzyme systems.
- To complement existing literature focused on cofactor regeneration chemistry.
- To classify sustainable cofactor regeneration strategies for industrial biotechnology.
Main Methods:
- Review of recent studies on cofactor retention in immobilized biocatalysts.
- Classification of cofactor regeneration methods into membrane entrapment and solid-attachment.
- Focus on methods facilitating cofactor recovery and reuse.
Main Results:
- Identified two primary categories for sustainable cofactor regeneration: membrane entrapment and solid-attachment.
- Highlighted the challenge of cofactor retention in immobilized enzyme systems.
- Emphasized the shift towards immobilized enzyme systems with cofactor regeneration since the late 1990s.
Conclusions:
- Effective cofactor retention is key to overcoming industrial hurdles in enzymatic biotransformations.
- Membrane entrapment and solid-attachment offer sustainable solutions for cofactor regeneration.
- Advancements in immobilized biocatalysts with cofactor regeneration are vital for industrial biotechnology's growth.
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