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Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
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Rab11 is associated with epidermal lamellar granules
Akemi Ishida-Yamamoto1, Mari Kishibe, Hidetoshi Takahashi
1Department of Dermatology, Asahikawa Medical College, Asahikawa, Japan. akemi@asahikawa-med.ac.jp
The Journal of Investigative Dermatology
|May 4, 2007
Summary
Rab11, a protein involved in intracellular transport, is found in epidermal lamellar granules (LGs). This suggests Rab11
Area of Science:
- Cell Biology
- Dermatology
- Molecular Biology
Background:
- Epidermal lamellar granules (LGs) are crucial for skin barrier function.
- LG molecule transport mechanisms are not fully understood.
- Defects in LGs cause severe skin diseases.
Purpose of the Study:
- To investigate the role of Rab11 in the intracellular trafficking of LG molecules.
- To determine the localization of Rab11 in normal human epidermis.
Main Methods:
- Confocal laser scanning microscopy was used to detect Rab11.
- Immunoelectron microscopy was employed for ultrastructural analysis.
- Colocalization studies with TGN46 and known LG molecules were performed.
Main Results:
- Rab11 immunoreactivity was observed in differentiated epidermal keratinocytes.
- Rab11 localized to the apical side of cells and colocalized with TGN46.
- Rab11 was found with various LG molecules, including lipids and proteins.
- Rab11 was distributed along the trans-Golgi network and tubular-vesicular structures.
Conclusions:
- Rab11 is involved in the transport of LG molecules.
- Rab11 likely plays a role in trafficking LG molecules from the TGN to the cell surface.
- This finding advances understanding of skin barrier formation and related diseases.
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