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Published on: July 10, 2021
Biomolecular papain thin films growth by laser techniques
Enikö György1, Jose Santiso, Albert Figueras
1National Institute for Lasers, Plasma and Radiations Physics, P.O. Box MG 36, Bucharest V 76900, Romania. eniko@ifin.nipne.ro
Summary
Pulsed laser deposition (PLD) was used to create papain thin films. Target preparation significantly impacts the biomaterial thin films
Area of Science:
- Biomaterials science
- Thin film deposition
- Laser-based material processing
Background:
- Papain, a proteolytic enzyme, has potential applications in biomaterials.
- Pulsed Laser Deposition (PLD) is a versatile technique for thin film synthesis.
Purpose of the Study:
- To synthesize papain thin films using two PLD methods.
- To investigate the influence of target preparation on film morphology and quality.
Main Methods:
- Matrix-assisted and conventional PLD techniques were employed.
- Targets were prepared from frozen biomaterial composites or pressed powders.
- An UV KrF* excimer laser at 0.5 J/cm² was used for ablation.
- Surface morphology was analyzed using atomic force microscopy (AFM) and profilometry.
Main Results:
- Both PLD methods successfully produced papain thin films.
- Target preparation significantly affected the growth mode and surface quality of the films.
- Lower laser fluence was required for biomaterials compared to inorganic materials.
Conclusions:
- The target preparation procedure is a critical factor in achieving desired papain thin film characteristics.
- PLD offers a viable route for fabricating enzyme-based biomaterial thin films.
