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Published on: December 19, 2020
[Synthesizing A beta(1-15) multiple antigen peptide vaccine by indirect conjugation and its immunological activity]
Xue-sen Yang1, Hua-qiao Wang, Zhi-bin Yao
1Department of Anatomy and Brain Research, Zhongshan School of Medicine, SUN Yat-sen University, Guangzhou 510080, China. xuesenyyy@yahoo.com.cn
Aim:
To explore the method of synthesizing the A beta(1-15) multiple antigen peptide (MAP) vaccine and to identify its quality and the immunological activity.
Methods:
MAP A beta(1-15) was synthesized by indirect conjugation and analyzed by RP-HPLC,SDA-PAGE and amino acid analysis. Then, C57BL/6 mice were immunized with synthesized MAP A beta(1-15). The specific anti-A beta antibody in the sera of the immunized mice was identified by ELSA.
Results:
There was a high and wide peak wave in the RP-HPLC chromatogram. The 8 protein bands identified by SDA-PAGE was identical with 1 to 8 branch of MAP A beta(1-15). The amino acid sequence of synthesized MAP A beta(1-15) was almost similar with the standard. High titer of anti A beta antibody was obtained in the C57BL/6 mice immunized with MAP A beta(1-15).
Conclusion:
MAP A beta(1-15) could be synthesized successfully by indirect conjugation and the synthesized MAP A beta(1-15) had satisfactory immune activity. But the purification of the synthesized complex remained to be a problem.
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