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Updated: Jul 15, 2026
Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats
Published on: April 7, 2023
[Isolation, cultivation of adipose-derived mesenchymal stem cells and its chondrogenic ability]
1Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Aim:
To find a method for isolation and cultivation of human adipose-derived mesenchymal stem cells(ADSCs), and to investigate its chondrogenic ability.
Methods:
Liposuction human adipose tissues were digested with collagenase type II. The obtained cells were primarily cultured in DMEM medium, and then changed into the induced medium. Alcian Blue stain, picrosirius red stain and the specific collagenase type II immunohistochemical staining were used to detect the results.
Results:
After cultured in chondrogenic medium, the cells differentiated toward cartilage cells and secreted the specific cartilaginous matrices sulfated proteglycan and collagen type II.
Conclusion:
The human ADSCs have the potential ability to differentiate toward cartilage cells in the appropriated medium. The result provide a new method for the further research in cartilaginous tissue engineering.
