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Updated: Aug 16, 2026
Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae
Published on: October 8, 2013
Low cost device for electrotransformation and its application to the highly efficient transformation of Escherichia
S Fujimoto1, H Hashimoto, Y Ike
1Department of Microbiology, School of Medicine, Gunma University, Maebashi, Japan.
Abstract:
A simple, low cost device for electrotransformation has been designed and constructed. The cost of the circuit was only $150. Maximum field strength of 12,000 V/cm with an actual time constant up to 11 msec was obtained with a newly designed circuitry and a 0.1-cm electrode gap cuvette. Eschericia coli strains DH1, DH5 alpha, and LE392 were transformed at an efficiency of 10(9)/micrograms DNA with plasmids pUC18 and pBR322. E. faecalis strain OG1X was transformed at an efficiency of 0.9 x 10(5)/micrograms DNA without treatment with glycine.
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