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Updated: Jul 14, 2026
08:40
Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
Published on: January 20, 2022
Ion mobility spectrometry-mass spectrometry performance using electrodynamic ion funnels and elevated drift gas
Erin Shammel Baker1, Brian H Clowers, Fumin Li
1Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99352, USA.
Summary
Higher resolution ion mobility spectrometry-mass spectrometry (IMS-MS) was achieved by using high-pressure ion funnels and elevated drift cell pressure. This advancement improves the characterization of complex biological mixtures.
Area of Science:
- Analytical Chemistry
- Biophysical Chemistry
Background:
- Ion mobility spectrometry coupled with mass spectrometry (IMS-MS) is valuable for analyzing biological mixtures.
- Existing IMS-MS methods face challenges with the complexity of biological samples, necessitating higher resolution.
Purpose of the Study:
- To develop a higher resolution IMS-MS instrument for improved characterization of complex biological samples.
- To enhance the performance of IMS-MS by optimizing ion funnel and drift cell parameters.
Main Methods:
- Developed a higher resolution electrospray ionization-ion mobility spectrometry-time-of-flight mass spectrometer (ESI-IMS-TOF MS).
- Utilized high-pressure electrodynamic ion funnels at both ends of the IMS drift cell.
- Operated the drift cell at elevated pressures (4-12 torr) and optimized drift voltages.
Main Results:
- Achieved an effective drift region of 98 cm by integrating the rear ion funnel.
- Increased instrument resolution from 55 to 80 by increasing pressure from 4 to 12 torr.
- Observed no significant loss in sensitivity with increased resolution and pressure.
Conclusions:
- The developed ESI-IMS-TOF MS offers significantly higher resolution for analyzing complex biological mixtures.
- High-pressure ion funnels and elevated drift cell pressures are effective strategies for enhancing IMS-MS performance.
- Further optimization of drift gas choice can influence ion behavior and trapping efficiency.
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