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Dual-labeled oligonucleotide probe for sensing adenosine via FRET: a novel alternative to SNPs genotyping

Yoshio Saito1, Subhendu Sekhar Bag, Yuichi Kusakabe

  • 1Department of Materials Chemistry and Engineering, School of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan. saitoy@chem.ce.nihon-u.ac.jp

Chemical Communications (Cambridge, England)
|May 24, 2007
PubMed

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...