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Related Experiment Video

Updated: Jun 18, 2026

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
11:53

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Published on: July 1, 2014

CILAT--a new reagent for quantitative proteomics.

Shuwei Li1, Dexing Zeng

  • 1Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, MD 20850, USA. liw@umbi.umd.edu

Chemical Communications (Cambridge, England)
|May 24, 2007
PubMed
Summary
This summary is machine-generated.

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Researchers developed a new quantitative proteomics reagent, Cleavable Isobaric Labeled Affinity Tag (CILAT). CILAT improves upon existing Isotope-Coded Affinity Tag (ICAT) and Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) methods.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Quantitative proteomics is crucial for understanding biological systems.
  • Existing methods like ICAT and iTRAQ have limitations in sensitivity and scope.
  • There is a need for improved reagents for accurate protein quantification.

Purpose of the Study:

  • To develop a novel reagent for enhanced quantitative proteomics.
  • To introduce Cleavable Isobaric Labeled Affinity Tag (CILAT) as an advancement.
  • To provide a superior alternative to current ICAT and iTRAQ methodologies.

Main Methods:

  • Development of the CILAT reagent.
  • Affinity-based enrichment of peptides.
  • Isobaric labeling for mass spectrometry-based quantification.

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Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
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Last Updated: Jun 18, 2026

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
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Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Published on: July 1, 2014

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
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Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay

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  • Comparative analysis with ICAT and iTRAQ.

    • Main Results:

    • CILAT demonstrates improved performance in quantitative proteomics.
    • The reagent offers enhanced sensitivity and specificity.
    • Successful application in complex biological samples.

    Conclusions:

    • CILAT represents a significant advancement in quantitative proteomics.
    • The new reagent offers a more robust and accurate approach for protein analysis.
    • CILAT has the potential to facilitate new discoveries in biological research.