Related Experiment Video
Updated: Jul 14, 2026

An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants
Published on: June 12, 2026
[Determination of monotropein in Radix Morindae from different processed products by HPLC]
Ji-yin Xu1, Ying-jiao Liang, Ping Ding
1College of Chinese Materia Medica, Guangzhou University of TCM, Guangzhou 510405, China. twtyfkfqvey@126.com
Objective:
To determine the contents of monotropein in the different processed Products of Radix Morindae.
Conditions:
Kromasil C18 (150 mm x 4. 6 mm,5 micorm) column; The mobile phase was methol-0.4% phosphate solution (5:95-->28.8: 71.2, 15 min); The velocity of flow was 1 ml/min; The detection wavelength was at 210 nm; And column temperature was 25 degrees C RESULTS: There were obvious differences between the crude and kinds of different processed products of monotropein in the Radix Morindae, The contents of monotropein from the different processed products (crude drugs, removed from wood, processed with salt, prepared) were 13.92 mg/g,9.10 mg/g, 9.21 mg/g, 12.86 mg/g, respectively.
Conclusion:
The different processing methods can make the contents of monotropein in the Radix Morindae.
More Related Videos
07:29HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
09:36Applications of Liquid-Chromatography Tandem Mass Spectrometry in Natural Products Research: Tropane Alkaloids as a Case Study
Published on: March 8, 2024