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Updated: Jul 14, 2026
Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation
Published on: April 3, 2026
[Discovery of rat Islet-1 gene variant and its expression in NSC]
Jia-mei Liu1, Dong Chen, Xiao-ting Meng
1Department of Histology and Embryology, School of Basic Medical Science, Jilin University, Changchun 130021, China. liujiamei100@yahoo.com.cn
Aim:
To construct rat Islet-1 gene recombinant retroviral expression vector and to transduce Islet-1 gene into neural stem cell (NSC) by the vector.
Methods:
The cDNA encoding the rat Islet-1 gene was isolated by RT-PCR method, the amplified gene fragment was subcloned into the retroviral vector plEGFP-C1. Islet-1 gene was transduced into NSC by PA317 packaging cells, then the expression of Islet-1 in NSC was observed.
Results:
The recombinant Islet-1 retroviral vector was constructed successfully, as shown by PCR, restriction enzymes digestion and fluorescence detection, and the positive expression of Islet-1 was found in NSC by immunocytochemical staining. The variant of Islet-1 gene was also discovered in this study.
Conclusion:
The rat Islet-1 recombinant retroviral vector has been successfully prepared, which can be used to study the function of Islet-1 gene in NSC differentiation into cholinergic neurons.
