[Procedure for extracting polyphosphoinositides from rat liver tissue and peculiarities of their metabolism]

The content of the rat liver triphosphoinositides and diphosphoinositides was determined in fresh tissue by the extraction with acid chloroform methanol mixture. The content was 7.7 +/- 0.5 mug P and 6.4 +/- 0.5 mug per 1 g of the tissue for triphosphoinositide and diphosphoinositides, respectively. The rapid enzymatic posthumorus changes are shown for the phospholipid content in the liver tissue; 5 min after the amount of triphosphoinositides increased by 19%, diphosphoinositides -- by 13% and that of total phospholipids -- by 11%; subsequently after 30 min after the content of di- and triphosphoinositides decreases below the initial values and that of other phospholipids returns to the initial level. The rate of 32P incorporation into polyphosphoinositide fractions increases rapidly during 1 hour and considerably exceeds that of all other phospholipid fractions studied.