Related Experiment Video
Updated: Jul 14, 2026
09:46
Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
Dissolution of bio-active dentine matrix components by mineral trioxide aggregate
Phillip L Tomson1, Liam M Grover, Philip J Lumley
1Oral Biology, School of Dentistry, The University of Birmingham, St Chad's Queensway, Birmingham B4 6NN, UK. p.l.tomson@bham.ac.uk
Journal of Dentistry
|June 15, 2007
Summary
Mineral trioxide aggregate (MTA) and calcium hydroxide solutions release dentine matrix proteins and signaling molecules. These components may influence cellular events crucial for dentine repair and regeneration.
Area of Science:
- Biomaterials Science
- Dental Pulp Biology
- Regenerative Medicine
Background:
- Mineral trioxide aggregate (MTA) is a widely used dental material for pulp capping and root-end filling.
- Understanding the soluble components released by MTA and their biological effects is crucial for optimizing its regenerative potential.
- Dentine matrix proteins (DMPs) and signaling molecules play key roles in pulpal healing and regeneration.
Purpose of the Study:
- To analyze soluble components released by setting and set MTA.
- To assess the ability of MTA and calcium hydroxide (Ca(OH)2) solutions to solubilize DMPs.
- To determine if these extracts contain signaling molecules vital for pulpal repair and regeneration.
Main Methods:
- Metallic ion composition analysis using atomic absorption spectroscopy.
- Extraction of dentine matrix components using MTA, Ca(OH)2, and EDTA solutions over 14 days.
- Analysis of extracts for non-collagenous proteins (NCPs), glycosaminoglycans (GAGs), and specific signaling molecules (TGF-β1, adrenomedullin) via 1D-PAGE and ELISA.
Main Results:
- MTA and Ca(OH)2 solutions released NCPs and GAGs, though in lower yields than EDTA.
- Distinct protein profiles were solubilized by different solutions.
- All extracts contained TGF-β1 and adrenomedullin (ADM); Ca(OH)2 and grey MTA released more ADM, while EDTA released more TGF-β1.
Conclusions:
- Soluble components released by setting and set MTA can liberate dentine matrix components.
- These released components may influence cellular events involved in dentine repair and regeneration.
- MTA's potential in regenerative endodontics is supported by its ability to release bioactive signaling molecules.
Related Concept Videos
01:31Essential Minerals for Bone Health
The minerals contained in all of the food we consume are essential for our organ systems. However, certain essential minerals, such as calcium, phosphorus, magnesium, manganese, and fluoride, largely affect bone health.
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
01:18The Bone Matrix
Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide an adherent surface for inorganic salt crystals. Both components of the matrix, organic and inorganic, contribute to the unusual properties of bone. Without collagen, bones would be brittle and shatter easily. Without mineral crystals, bones would flex and provide little support. This can be observed by an experiment: when the minerals of a bone are dissolved by soaking the bone in acid or...