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[The effect of cyrstal water on determining content of baicalin]
Hou-quan Wang1, Gang Ni, Hui-xia Ren
1College of Pharmacy, Shandong University, Jinan 250012, China.
Objective:
To explore the effect of crystal water on deteriming content of baicalin.
Methods:
Baicalin samples containing different amount of crystal water were determined by HPLC.
Results:
The determining results of baicalin were accurate and stable on condition that the samples contain some crystal water or some impurities, and the determining results of baicalin were reduced remarkably on condition that the samples are anhydrous and pure.
Conclusion:
The determining results of baicalin contents were accurate and stable only if the oxygen in 1 place forms hydrogen bond, and the determining results of baicalin contents were reduced remarkably on condition that the oxygen in 1 place forms protonated ether of form positive carbonic.
