[Comparison of biochemical criteria for supplying the human body with riboflavin]

Various biochemical procedures were used in evaluation of vitamin B2 occurrence in 38 women. Highly specific procedures for evaluation of riboflavin in blood and urine (high performance liquid chromatography, titration with riboflavin-binding apoprotein, lumiflavin procedure) enabled to detect correlation between values of FAD-effect and excretion of riboflavin with urine as well as of total riboflavin content in erythrocytes, between concentration of free riboflavin in blood plasma and its excretion with urine. All the procedures studied were equally suitable for evaluation of vitamin B2 deficiency as shown by the data obtained as well as by similarity in values of low concentrations which corresponded to normal availability of the vitamin.