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Cost-Efficient Transcriptomic-Based Drug Screening
Published on: February 23, 2024
Cost effective method for construction of high quality cDNA libraries
Andreia Figueiredo1, Aladje Baldé, Hélia Cardoso
1Unit of Molecular Biology and Plant Biotechnology-ICAT (Institute for Applied Science and Technology) Ed. ICAT, Campo Grande, 1749-016 Lisbon, Portugal. andreia.figueiredo@icat.fc.ul.pt
Biomolecular Engineering
|July 10, 2007
Abstract:
A PCR-based method for the synthesis of high quality cDNA is described. This method combines the reverse transcriptase template switching with the assisted recombination cloning. It enables 5' end enrichment, cDNA synthesis from limiting starting material, elimination of digestion and subcloning steps and substantial time and cost savings.
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