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[hTERT promoter regulated tumor targeting TRALL in cervical cancer cell]
Ming Zhang1, Xiao-yan Xin, Hong-mei Li
1Department of Obstetrics and Gynecology, Fourth Military Medical University, Xi'an 710032, China.
Aim:
To investigate the effect of hTERT promoter which regulated tumor targeting TRALL on the cervical cancer cell line HeLa.
Methods:
The mRNA expression of TRAIL on HeLa was examined by RT-PCR. The proliferation, apoptosis, motion of the transfected cervical cancer cell were detected by MTT, flow cytometry, and cell invasion assay respectively. The ultrastructure was observed by electron microscope.
Results:
Compared with the control group, the expression of TRAILmRNA was significantly upregulated in hTERT-TRAIL (P<0.05). The apotosis rate and the inhibitory rate of cell growth of hTERT-TRAIL was significantly high (P<0.05). Electron microscope results indicated that hTERT promoter regulated tumor targeting TRALL facilitated the apoptosis of HeLa.
Conclusion:
The hTERT-TRAIL significantly inhibits the malignant proliferation and invasion ability of the cervical cancer cell line, and facilitates the apoptosisof HeLa, which lays a foundation for the treatment of patients with cervical cancer.
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