[Construction and identification of an immune single chain antibody library derived from a chicken]
1Key Laboratory of Food Science of Ministry of Education, Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China. gjychb@yahoo.com.cn
Aim:
To develop a method for constructing an immune single chain antibody library derived from a chicken.
Methods:
According to the principle of phage display and the character of chicken antibody genes, an immune single chain antibody library was constructed by using the spleen B cells which were extracted from a chicken immunized with streptococcus mutans as antibody gene sources. According to the principle of immobilized panning the specific chicken single chain phage antibody was selected from the single chain antibody library to identify the immune antibody library.
Results:
The repertoire of the chicken heavy-chain and light-chain variable regions was obtained from the total intact RNA of chicken spleen. The phage antibody library was generated and its size was determined to be 1.6x10(7). A specific chicken single chain phage antibody against streptococcus mutans was selected and its DNA was sequenced.
Conclusion:
An immune single chain antibody library derived from a chicken can be constructed successfully based on the principle of phage antibody library and the character of chicken antibody genes.
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