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Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry
Published on: March 1, 2020
Surface analysis under ambient conditions using plasma-assisted desorption/ionization mass spectrometry.
Lucy V Ratcliffe1, Frank J M Rutten, David A Barrett
1Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom.
Analytical Chemistry
|July 14, 2007
Summary
A new plasma-assisted desorption/ionization (PADI) method enables rapid mass spectrometry analysis of various surfaces without sample prep. This technique shows potential for high-throughput pharmaceutical and forensic screening.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Plasma Physics
Background:
- Traditional mass spectrometry often requires extensive sample preparation, limiting high-throughput analysis.
- Ambient ionization techniques aim to simplify sample handling and analysis.
- Existing methods like desorption electrospray ionization (DESI) have limitations.
Purpose of the Study:
- To introduce and demonstrate a novel plasma-assisted desorption/ionization (PADI) technique.
- To evaluate PADI's capability for analyzing diverse samples under ambient conditions.
- To compare PADI with DESI for pharmaceutical analysis.
Main Methods:
- Generated a nonthermal plasma for direct surface interaction with analytes.
- Utilized atmospheric pressure sampling mass spectrometry for ion detection.
- Interfaced a prototype PADI source with LCZ and HPR-60 mass spectrometers.
Main Results:
- Successfully detected active pharmaceutical ingredients (NSAIDs, analgesics) from various formulations.
- Analyzed nicotine in tobacco and thiosulfates in garlic using PADI.
- Demonstrated PADI's effectiveness without sample preparation, solvents, or high voltages.
Conclusions:
- PADI is a versatile ambient ionization technique for mass spectrometry.
- The method allows rapid analysis of diverse analytes directly from surfaces.
- PADI holds significant potential for high-throughput screening in pharmaceutical and forensic applications.
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