Related Experiment Video
Updated: Jul 12, 2026

The Effect of Interfacial Chemical Bonding in TiO2-SiO2 Composites on Their Photocatalytic NOx Abatement Performance
Published on: July 4, 2017
Mapping the conformational landscape of urea-denatured ubiquitin using residual dipolar couplings
Sebastian Meier1, Stephan Grzesiek, Martin Blackledge
1Biozentrum, University of Basel, Klingelbergstrasse 50, 4056 Basel, Switzerland.
Abstract:
Characterization of the unfolded state is a fundamental prerequisite for understanding protein stability and folding. We have investigated local conformational sampling in urea-denatured ubiquitin at pH 2.5 by measuring an extensive set of residual dipolar couplings (RDCs) under conditions of partial molecular alignment. Seven experimental RDCs per peptide unit, including complementary fixed-geometry and interproton (1H(N)-1H(N) and 1H(N)-1H(alpha)) couplings, were used to investigate the structural properties of the peptide chain. Amino-acid-specific potentials that simultaneously reproduce all RDCs in the molecule are found to sample more extended conformations than the standard statistical coil description. Analysis of 3J(HNHalpha) scalar couplings measured under the same conditions suggests that neither polyproline II nor extended beta-regions dominate this additional sampling of extended conformations. Using this approach we propose a model of the conformational landscape throughout the peptide chain of urea-denatured ubiquitin, providing an essential description for understanding the unfolded state.
More Related Videos
10:27Simultaneous Multi-surface Anodizations and Stair-like Reverse Biases Detachment of Anodic Aluminum Oxides in Sulfuric and Oxalic Acid Electrolyte
Published on: October 5, 2017
08:02Rendering SiO2/Si Surfaces Omniphobic by Carving Gas-Entrapping Microtextures Comprising Reentrant and Doubly Reentrant Cavities or Pillars
Published on: February 11, 2020
Related Concept Videos
Dehydration of Aldols to Enals: Base-Catalyzed Aldol Condensation
Dehydration of Aldols to Enones: Acid-Catalyzed Aldol Condensation
Ladder Diagrams: Complexation Equilibria
The formation constant, K1, for the formation of Cd(NH3)2+ complex from cadmium and ammonia is 3.55 × 102. Log K1 (i.e. pNH3) is 2.55, and...
EDTA: Auxiliary Complexing Reagents
Formation of Dilute Urine
Filtrate Osmolarity in the PCT
Initially, as the filtrate passes through the proximal convoluted tubule (PCT), its...
Formation of Concentrated Urine