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Engineering thick tissues--the vascularisation problem.

Henry C H Ko1, Bruce K Milthorpe, Clive D McFarland

  • 1Graduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW, Australia.

European Cells & Materials
|July 27, 2007
PubMed
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Creating thick engineered tissues requires a vascular supply. Researchers are developing in vitro vascular networks using cell-seeded scaffolds and biomaterials to improve tissue viability after implantation.

Area of Science:

  • Tissue Engineering
  • Biomaterials Science
  • Vascular Biology

Background:

  • Engineering thick tissues is limited by the challenge of creating adequate vascularization.
  • Current strategies involve seeding cells into hybrid matrix/scaffold systems to form in vitro vascular analogues.
  • Developing functional vascular networks is crucial for cell survival in engineered tissues.

Purpose of the Study:

  • To review and discuss approaches for creating in vitro vascular supplies for engineered tissues.
  • To highlight methods enabling seamless integration of engineered tissues with host vasculature.
  • To address the challenge of maintaining cell viability in thick, bioengineered constructs.

Main Methods:

  • Utilizing biological extracellular matrices like collagen hydrogels.

Related Experiment Videos

  • Employing porous biodegradable polymeric scaffolds with defined lumen and channel structures.
  • Incorporating cell co-cultures, growth factors, and dynamic bioreactor environments.

    • Main Results:

    • Various in vitro methods show promise for generating vascular networks within engineered tissues.
    • Combinations of different approaches may enhance vascularization efficiency.
    • Focus on pre-vascularized constructs facilitates host vasculature integration.

    Conclusions:

    • Successful vascularization of engineered tissues is key to overcoming the challenge of creating thick constructs.
    • In vitro vascular analogue development is critical for future clinical applications.
    • Strategies enabling direct connection to host vasculature post-implantation are essential for long-term tissue survival.