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Fabrication and Testing of Microfluidic Optomechanical Oscillators

Fabrication and Testing of Microfluidic Optomechanical Oscillators

Published on: May 29, 2014

New technology for cellular piercing: rotationally oscillating mu-injector, description and validation tests.

Ali Fuat Ergenc¹, Nejat Olgac

¹Mechanical Engineering Department, University of Connecticut, 191 Auditorium Rd EII Build, Unit 3139, Storrs, CT 06269, USA.

Biomedical Microdevices

|July 31, 2007

Summary

A novel mercury-free microinjection device, the Ros-Drill, uses rotational oscillations for effective cell piercing. This minimally invasive technique improves intracytoplasmic sperm injection (ICSI) success rates by reducing damaging pipette movements.

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Single-cell Microinjection for Cell Communication Analysis

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Published on: February 26, 2017

Area of Science:

Reproductive biology
Biomedical engineering
Cellular biology

Background:

Intracytoplasmic sperm injection (ICSI) is a common assisted reproductive technology.
Current ICSI methods use piezo actuators and mercury, which can cause damaging pipette oscillations and cell membrane damage.
These oscillations negatively impact ICSI success rates.

Purpose of the Study:

To introduce a novel, mercury-free microinjection procedure to overcome the limitations of current ICSI techniques.
To develop and test a new device, the Ros-Drill, that utilizes rotational oscillations for improved cell piercing.

Main Methods:

A new microinjection device, the Ros-Drill, was designed and built, incorporating rotational oscillations.
The device utilizes high-frequency (≥100 Hz) rotational oscillations with small amplitudes (few degrees).
Prototypes included peripheral control hardware and software for implementation.

Main Results:

The Ros-Drill demonstrated effective cell piercing using rotational oscillations.
Preliminary experiments on mouse oocytes showed encouraging results.
Several mouse oocytes developed to the blastocyst stage using the new drilling device.

Conclusions:

The Ros-Drill offers a mercury-free and minimally invasive alternative for microinjection procedures.
Rotational oscillations are effective for cell piercing, potentially enhancing ICSI success rates.
The developed technology and its implementation protocols show promise for future applications in reproductive medicine.