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Updated: Jul 13, 2026
10:11
Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer
Published on: April 19, 2021
Characterization of a temperature-controlled FAIMS system.
David A Barnett1, Michael Belford, Jean-Jacques Dunyach
1Thermo Fisher Scientific, San Jose, California, USA.
Journal of the American Society for Mass Spectrometry
|July 31, 2007
Summary
High-field asymmetric waveform ion mobility spectrometry (FAIMS) performance is temperature-dependent. A new FAIMS system with gas heating/cooling offers improved ion separation and detection in biological samples.
Area of Science:
- Analytical Chemistry
- Separation Science
- Mass Spectrometry
Background:
- High-field asymmetric waveform ion mobility spectrometry (FAIMS) enhances analyte detection in biological matrices.
- Conventional FAIMS performance is significantly impacted by heat from ionization sources and MS interfaces.
- Standard LC-MS workflows often require elevated temperatures for ion desolvation, affecting FAIMS performance.
Purpose of the Study:
- To introduce a novel FAIMS system with integrated gas heating/cooling for temperature control.
- To investigate the impact of temperature on ion behavior and separation within FAIMS.
- To demonstrate how temperature control can manipulate ion separation for improved analytical outcomes.
Main Methods:
- Development of a new FAIMS system with independent gas heating/cooling capabilities.
- Application of theoretical equations and balance plots to analyze normalized field strength (E/N) under varying temperatures.
- Experimental observation and explanation of ion behavior, including deviations attributed to ion clusters and ion-neutral interactions.
Main Results:
- The new FAIMS system achieves rapid temperature equilibrium, independent of external conditions.
- Temperature significantly affects ion mobility and separation, with deviations from predicted behavior observed.
- Differential temperature settings on inner and outer electrodes were shown to effectively manipulate ion separation.
Conclusions:
- Temperature control is crucial for optimizing FAIMS performance and ion separation.
- The novel temperature-controlled FAIMS system offers enhanced control over ion mobility.
- This technology provides advantages for targeted species detection in complex biological matrices.
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