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Updated: Jul 13, 2026

Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins
Published on: May 27, 2019
The structure of the exocellular polysaccharide produced by Rhodococcus sp. RHA1
Malcolm B Perry1, Leann L MacLean, Marianna A Patrauchan
1Institute for Biological Sciences, National Research Council, Ottawa, Canada K1A 0R6. malcolm.perry@nrc-cnrc.gc.ca
Abstract:
Rhodococcus sp. RHA1 is a Gram-positive actinomycete capable of metabolizing a wide spectrum of organic compounds whose survival in chemically hostile environments is believed to be in part due to the production of an exocellular polysaccharide (EPS). In order to investigate the functional nature of the EPS, its structure was determined using a combinatory approach including hydrolysis, composition, and methylation, analysis methods, as well as 2D (1)H and (13)C NMR spectroscopy. The EPS was found to be a high-molecular-mass polymer of a repeating tetrasaccharide unit composed of D-glucuronic acid, D-glucose, D-galactose, L-fucose and O-acetyl (1:1:1:1:1), and has the structure:
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