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[Studies on chemical constituents of Patrinia villosa]
Long Huang1, Ru-song Zhang, Cai-fang Wang
1Zheng Dai Qing Chun Bao Pharmacy Co. Ltd, Huangzhou, China.
Objective:
To inverstigate the chemical constituents of Patrinia villosa.
Method:
Under the direction of tetrazolium salt (MTf) assay, the chemical constituents were isolated by silica gel column chromatography and identified by physicochemical properties and spectral analysis (MS, FAB-MS, 1H NMR, 13C NMR).
Result:
Five compounds were isolated from petroleum ether and ethyl acetate extract and identified as: ursolic acid(I), beta-sitosterol (II), patrinalloside(III), kaempferol-3-O-beta-D-glucopyranoside(IV), and kaempferol-3-O-beta-D-glucopyranoside-7-O-alpha-L-rhamnoside(V).
Conclusion:
Compounds I and V were obtained from the plant of genus Patrinia for the first time. Ursolic acid showed powerful anticancer activity through tetrazolium salt(MTT) assay, and its IC50 was 15.0 microg/ml.
