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Fluoride balance and tissue concentrations: effect of dose frequency
G M Whitford1, N L Birdsong-Whitford, J M Augeri
1Department of Oral Biology, School of Dentistry, Medical College of Georgia, Augusta.
Proceedings of the Finnish Dental Society. Suomen Hammaslaakariseuran Toimituksia
|January 1, 1991
Summary
Smaller, more frequent fluoride doses had minor effects on absorption and retention in rats. Dose frequency minimally impacts fluoride bioavailability and tissue levels when intake occurs daily.
Area of Science:
- Biochemistry
- Pharmacokinetics
- Toxicology
Background:
- Fluoride intake and its distribution in the body are influenced by various factors.
- The role of dose frequency in fluoride bioavailability and tissue retention requires further investigation.
Purpose of the Study:
- To investigate the impact of fluoride dose frequency on bioavailability, balance, and tissue levels in young adult rats.
- To test the hypothesis that more frequent, smaller fluoride doses increase fluoride retention due to exchangeable pools in calcified tissues.
Main Methods:
- Young adult rats were divided into groups receiving fluoride via diet, ad libitum water, daily oral intubation, or daily intraperitoneal injection over six weeks.
- Fluoride intake and excretion were measured over 24-hour periods weekly.
- Plasma, enamel, and femur epiphysis fluoride levels were analyzed at study termination.
Main Results:
- Groups receiving more frequent doses (oral or intraperitoneal) showed slightly higher average fluoride absorption (13%) and balance (10%) compared to ad libitum water intake.
- Plasma and calcified tissue fluoride levels were marginally elevated in more frequently dosed groups, but differences were not statistically significant.
- Intraperitoneal injection led to a 96% greater fecal excretion than dietary intake, indicating net intestinal secretion.
Conclusions:
- Dose frequency has minimal impact on fluoride absorption, balance, and tissue levels in rats for a given intake level.
- The influence of the exchangeable calcified tissue fluoride pool on overall fluoride metabolism is limited when intake occurs at least once daily.