Folding and misfolding of the collagen triple helix: Markov analysis of molecular dynamics simulations
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Updated: Jul 12, 2026

Microfluidic Mixers for Studying Protein Folding
Published on: April 10, 2012
Sanghyun Park1, Teri E Klein, Vijay S Pande
1Department of Chemistry, Stanford University, Stanford, California, USA.
Abstract:
Folding and misfolding of the collagen triple helix are studied through molecular dynamics simulations of two collagenlike peptides, [(POG)(10)](3) and [(POG)(4)POA(POG)(5)](3), which are models for wild-type and mutant collagen, respectively. To extract long time dynamics from short trajectories, we employ Markov state models. By analyzing thermodynamic and kinetic quantities calculated from the Markov state models, we examine folding mechanisms of the collagen triple helix and consequences of glycine mutations. We find that the C-to-N zipping of the collagen triple helix must be initiated by a nucleation event consisting of formation of three stable hydrogen bonds, and that zipping through a glycine mutation site requires a renucleation event which also consists of formation of three stable hydrogen bonds. Our results also suggest that slow kinetics, rather than free energy differences, is mainly responsible for the stability of the collagen triple helix.
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