Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Wide dynamic range phase-sensitive surface plasmon resonance biosensor based on measuring the modulation harmonics.

Wing-Cheung Law1, Przemyslaw Markowicz, Ken-Tye Yong

  • 1Institute for Lasers, Photonics and Biophotonics, Departments of Electrical Engineering, State University of New York at Buffalo, Buffalo, NY 14260, USA.

Biosensors & Bioelectronics
|September 7, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrating Artificial Intelligence with Ramanomics for Label-Free Monitoring of Biochemical Environment in Live Cells to Advance Cellular Diagnostics and Molecular Medicine.

ACS omega·2026
Same author

Sulfonamide DPA-Based Mononuclear Cobalt(II) Complexes: Evaluation of ROS-Driven Cytotoxicity.

Chemistry, an Asian journal·2026
Same author

Photonic cavity and graphene modified plasmonic interface for enhanced sensing performance.

Talanta·2026
Same author

Probing into the binding mechanism of phosphorus(V)-corrole with hemoglobin using photophysical and computational approach.

Scientific reports·2026
Same author

Strain-Responsive and Self-Healing Chiral Polymer Vitrimer Composites: Tunable Circular Dichroism and Mechanochromic Recovery in Self-Standing Films.

ACS applied materials & interfaces·2026
Same author

Deep learning-enabled high-performance multiphoton fluorescence vascular imaging using clinically approved fluorescent probes.

iScience·2026

This study introduces a novel phase-sensitive surface plasmon resonance (SPR) setup for sensitive biomolecular interaction analysis. The system achieves ultra-high phase sensitivity and a low detection limit for biosensing applications.

Area of Science:

  • Analytical Chemistry
  • Biophysics
  • Materials Science

Background:

  • Surface Plasmon Resonance (SPR) is a label-free optical technique widely used for studying biomolecular interactions.
  • Traditional SPR setups often face limitations in sensitivity and dynamic range.
  • Developing advanced SPR systems is crucial for enhancing biosensing capabilities.

Purpose of the Study:

  • To develop a novel phase-sensitive surface plasmon resonance (SPR) setup.
  • To achieve ultra-high phase sensitivity and a wide dynamic range for biomolecular interaction studies.
  • To demonstrate the system's performance in real-time biosensing applications.

Main Methods:

  • A novel phase-sensitive SPR setup was designed using temporal modulation of a pumping beam.

Related Experiment Videos

  • A photoelastic modulator was employed for beam modulation.
  • Phase information was extracted at the second and third harmonics of the modulation frequency.
  • The setup was tested using biotin-protein and streptavidin-bovine serum albumin (BSA) complex interactions.

    • Main Results:

    • The developed SPR setup demonstrated ultra-high phase sensitivity.
    • A wide dynamic range of measurements was achieved.
    • A detection limit as low as 2.89 x 10^-7 refractive index units (RIU) was obtained.
    • A detection sensitivity of 1.3 nM was achieved for the streptavidin-maleimide/thiolated BSA complex binding reaction.

    Conclusions:

    • The novel phase-sensitive SPR setup offers superior performance for biomolecular interaction analysis.
    • The system provides a sensitive and reliable platform for real-time biosensing.
    • This advancement has significant implications for diagnostics and drug discovery.