Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple hybridization-extension sequencing (MHES) on microarray.

Zhiqiang Pan1, Yanqiang Li, Pengfeng Xiao

  • 1State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China.

Journal of Biochemistry
|September 11, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nickel/Photoredox-catalyzed diastereoselective semihydrogenation of alkynes using a carboxylic acid as a cooperative ligand and hydrogen surrogate.

Nature communications·2026
Same author

Highly efficient tribocatalytic hydrogen peroxide production over Fe<sub>2</sub>O<sub>3</sub> nanoparticles and the reaction pathways.

Journal of colloid and interface science·2026
Same author

TET1 downregulation in the dorsal root ganglion and spinal cord is required for short-term sleep disturbance to delay surgical pain recovery.

British journal of anaesthesia·2026
Same author

Phytotoxic activity of grammicin produced by the fungus Xylaria grammica (Ascomycota) recovered from infected lesions on Handroanthus serratifolius (Bignoniaceae) leaves.

Pest management science·2026
Same author

Increased dissolved methane production driven by phosphorus enrichment in rivers draining from varied climate zones to eastern China's coastal waters.

Water research·2026
Same author

Doped RuO<sub>2</sub> for Acidic Oxygen Evolution Reaction: From Transitional Metals, Main-Group Metals to Nonmetals.

ChemSusChem·2026
Same journal

Estrogen-related receptor γ functions as a novel corepressor of androgen receptor by destabilizing receptor conformation and blocking coactivator recruitment.

Journal of biochemistry·2026
Same journal

Chromatin dynamics and cellular energy homeostasis.

Journal of biochemistry·2026
Same journal

Nutrigenomic Regulation of One-Carbon Metabolism and the Circadian Clock in Health and Disease.

Journal of biochemistry·2026
Same journal

Time to rethink circadian rhythms beyond the transcriptome.

Journal of biochemistry·2026
Same journal

BNIP3/NIX-dependent mitophagy: molecular mechanisms and physiological roles.

Journal of biochemistry·2026
Same journal

Regulatory mechanism of heme-regulated inhibitor through autophosphorylation-driven activation and heme-induced deactivation.

Journal of biochemistry·2026
See all related articles

This study introduces multiple hybridization-extension sequencing (MHES), a novel method for DNA sequencing on microarrays. MHES overcomes limitations of traditional sequencing-by-synthesis by eliminating the need for fluorophore quenching, enabling longer reads.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Sequencing-by-synthesis (SBS) on microarrays faces challenges with low quenching efficiency, limiting read length.
  • Efficiently removing extended fluorophore-bases is crucial for accurate, long-read DNA sequencing.

Purpose of the Study:

  • To develop a new DNA sequencing strategy, multiple hybridization-extension sequencing (MHES), to overcome read-length limitations in SBS.
  • To eliminate the requirement for fluorophore quenching during the sequencing process.

Main Methods:

  • MHES involves primer hybridization to immobilized ssDNA templates on a microarray.
  • Sequencing of 3-5 bases using Cy5-dNTP, followed by lambda DNA exonuclease removal of extended primers.
  • Repeated hybridization and extension cycles, with polished bases using natural dNTPs, enable sequencing of unknown DNA fragments.

Related Experiment Videos

Main Results:

  • Accurate sequencing of a 10 bp fragment from a synthetic template.
  • Accurate sequencing of a 10 bp fragment from the DTBNP1 gene.
  • Demonstrated potential for increased read-length and high-throughput sequencing.

Conclusions:

  • MHES offers a promising alternative to conventional SBS for DNA sequencing on microarrays.
  • The method significantly improves read-length potential by removing the need for fluorophore quenching.
  • MHES is suitable for high-throughput sequencing applications.